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universal plus mrna-seq library construction kit  (Tecan Systems)


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    Tecan Systems universal plus mrna-seq library construction kit
    (A) Plk4 overexpression promotes centriole overduplication. Structured Illumination Microscopy (SIM) images of RPE-1 cells with endogenous and overexpressed Plk4. Plk4, magenta and centrioles, green. Scale bar 0.5 μm. (B) Unk is elevated upon Plk4 overexpression. Left panel – MA plot of <t>mRNA</t> levels based on RNA <t>seq</t> of RPE-1 cells with overexpressed Plk4, 16 hours after overexpression. PLK4 and UNK transcripts are elevated by 1.5- and 1.4-fold, respectively. Right panel – frequencies of PLK4 and UNK mRNAs in RPE-1 cells with endogenous and overexpressed Plk4. Graph values expressed as the means of biological replicates and SD. P values were determined using one-way ANOVA with Šídák post hoc test. (C) Unk protein is elevated at the centrosome by Plk4 overexpression. Left panels – SIM images of endogenous Unk in RPE-1 cells with endogenous and overexpressed Plk4. Unk, magenta and centrioles, green. Scale bar, 5 μm. Insets scale bar, 1 μm. Middle panels – 8 μm radial fluorescence intensity and corresponding ratio quantification of Unk using Centrin as the centrosome centroid. Right panel – centrosomal Unk fluorescence intensity based on binned central 2 μm. Graph values expressed as the means of biological replicates and SD. P value was determined using an unpaired two-tailed t test. (D) Unk is required for Plk4-induced centriole overduplication. Left panels – SIM images of RPE-1 cells showing centrioles in Unk depleted cells with endogenous and overexpressed Plk4. Unk, magenta and centrioles, green. Scale bar, 0.5 μm. Middle panel – frequency of cells with centriole overduplication. Right panel – number of cells with greater than, equal to, and less than four centrioles. Graph values expressed as the means of biological replicates and SD. P values were determined using one-way ANOVA with Šídák post hoc test.
    Universal Plus Mrna Seq Library Construction Kit, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/universal+plus+mrna-seq+library+construction+kit/universal+plus+mrna+kit/bio_rxiv__2024__07__29__605660-390-6-12
    Average 90 stars, based on 1 article reviews
    universal plus mrna-seq library construction kit - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "The Unkempt RNA binding protein reveals a local translation program in centriole overduplication"

    Article Title: The Unkempt RNA binding protein reveals a local translation program in centriole overduplication

    Journal: bioRxiv

    doi: 10.1101/2024.07.29.605660

    (A) Plk4 overexpression promotes centriole overduplication. Structured Illumination Microscopy (SIM) images of RPE-1 cells with endogenous and overexpressed Plk4. Plk4, magenta and centrioles, green. Scale bar 0.5 μm. (B) Unk is elevated upon Plk4 overexpression. Left panel – MA plot of mRNA levels based on RNA seq of RPE-1 cells with overexpressed Plk4, 16 hours after overexpression. PLK4 and UNK transcripts are elevated by 1.5- and 1.4-fold, respectively. Right panel – frequencies of PLK4 and UNK mRNAs in RPE-1 cells with endogenous and overexpressed Plk4. Graph values expressed as the means of biological replicates and SD. P values were determined using one-way ANOVA with Šídák post hoc test. (C) Unk protein is elevated at the centrosome by Plk4 overexpression. Left panels – SIM images of endogenous Unk in RPE-1 cells with endogenous and overexpressed Plk4. Unk, magenta and centrioles, green. Scale bar, 5 μm. Insets scale bar, 1 μm. Middle panels – 8 μm radial fluorescence intensity and corresponding ratio quantification of Unk using Centrin as the centrosome centroid. Right panel – centrosomal Unk fluorescence intensity based on binned central 2 μm. Graph values expressed as the means of biological replicates and SD. P value was determined using an unpaired two-tailed t test. (D) Unk is required for Plk4-induced centriole overduplication. Left panels – SIM images of RPE-1 cells showing centrioles in Unk depleted cells with endogenous and overexpressed Plk4. Unk, magenta and centrioles, green. Scale bar, 0.5 μm. Middle panel – frequency of cells with centriole overduplication. Right panel – number of cells with greater than, equal to, and less than four centrioles. Graph values expressed as the means of biological replicates and SD. P values were determined using one-way ANOVA with Šídák post hoc test.
    Figure Legend Snippet: (A) Plk4 overexpression promotes centriole overduplication. Structured Illumination Microscopy (SIM) images of RPE-1 cells with endogenous and overexpressed Plk4. Plk4, magenta and centrioles, green. Scale bar 0.5 μm. (B) Unk is elevated upon Plk4 overexpression. Left panel – MA plot of mRNA levels based on RNA seq of RPE-1 cells with overexpressed Plk4, 16 hours after overexpression. PLK4 and UNK transcripts are elevated by 1.5- and 1.4-fold, respectively. Right panel – frequencies of PLK4 and UNK mRNAs in RPE-1 cells with endogenous and overexpressed Plk4. Graph values expressed as the means of biological replicates and SD. P values were determined using one-way ANOVA with Šídák post hoc test. (C) Unk protein is elevated at the centrosome by Plk4 overexpression. Left panels – SIM images of endogenous Unk in RPE-1 cells with endogenous and overexpressed Plk4. Unk, magenta and centrioles, green. Scale bar, 5 μm. Insets scale bar, 1 μm. Middle panels – 8 μm radial fluorescence intensity and corresponding ratio quantification of Unk using Centrin as the centrosome centroid. Right panel – centrosomal Unk fluorescence intensity based on binned central 2 μm. Graph values expressed as the means of biological replicates and SD. P value was determined using an unpaired two-tailed t test. (D) Unk is required for Plk4-induced centriole overduplication. Left panels – SIM images of RPE-1 cells showing centrioles in Unk depleted cells with endogenous and overexpressed Plk4. Unk, magenta and centrioles, green. Scale bar, 0.5 μm. Middle panel – frequency of cells with centriole overduplication. Right panel – number of cells with greater than, equal to, and less than four centrioles. Graph values expressed as the means of biological replicates and SD. P values were determined using one-way ANOVA with Šídák post hoc test.

    Techniques Used: Over Expression, Microscopy, RNA Sequencing Assay, Fluorescence, Two Tailed Test

    Related Articles

    Construct:

    Article Title: The Unkempt RNA binding protein reveals a local translation program in centriole overduplication
    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.

    Article Title: The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis
    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.

    Microarray:

    Article Title: The Unkempt RNA binding protein reveals a local translation program in centriole overduplication
    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.

    Article Title: The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis
    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.

    Over Expression:

    Article Title: The Unkempt RNA binding protein reveals a local translation program in centriole overduplication
    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.

    Article Title: The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis
    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.

    Microscopy:

    Article Title: The Unkempt RNA binding protein reveals a local translation program in centriole overduplication
    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.

    Article Title: The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis
    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.

    RNA Sequencing Assay:

    Article Title: The Unkempt RNA binding protein reveals a local translation program in centriole overduplication
    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.

    Article Title: The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis
    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.

    Fluorescence:

    Article Title: The Unkempt RNA binding protein reveals a local translation program in centriole overduplication
    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.

    Article Title: The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis
    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.

    Two Tailed Test:

    Article Title: The Unkempt RNA binding protein reveals a local translation program in centriole overduplication
    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.

    Article Title: The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis
    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.



    Similar Products

    universal plus mrna-seq library construction kit  (Tecan Systems)
    90
    Tecan Systems universal plus mrna-seq library construction kit
    (A) Plk4 overexpression promotes centriole overduplication. Structured Illumination Microscopy (SIM) images of RPE-1 cells with endogenous and overexpressed Plk4. Plk4, magenta and centrioles, green. Scale bar 0.5 μm. (B) Unk is elevated upon Plk4 overexpression. Left panel – MA plot of <t>mRNA</t> levels based on RNA <t>seq</t> of RPE-1 cells with overexpressed Plk4, 16 hours after overexpression. PLK4 and UNK transcripts are elevated by 1.5- and 1.4-fold, respectively. Right panel – frequencies of PLK4 and UNK mRNAs in RPE-1 cells with endogenous and overexpressed Plk4. Graph values expressed as the means of biological replicates and SD. P values were determined using one-way ANOVA with Šídák post hoc test. (C) Unk protein is elevated at the centrosome by Plk4 overexpression. Left panels – SIM images of endogenous Unk in RPE-1 cells with endogenous and overexpressed Plk4. Unk, magenta and centrioles, green. Scale bar, 5 μm. Insets scale bar, 1 μm. Middle panels – 8 μm radial fluorescence intensity and corresponding ratio quantification of Unk using Centrin as the centrosome centroid. Right panel – centrosomal Unk fluorescence intensity based on binned central 2 μm. Graph values expressed as the means of biological replicates and SD. P value was determined using an unpaired two-tailed t test. (D) Unk is required for Plk4-induced centriole overduplication. Left panels – SIM images of RPE-1 cells showing centrioles in Unk depleted cells with endogenous and overexpressed Plk4. Unk, magenta and centrioles, green. Scale bar, 0.5 μm. Middle panel – frequency of cells with centriole overduplication. Right panel – number of cells with greater than, equal to, and less than four centrioles. Graph values expressed as the means of biological replicates and SD. P values were determined using one-way ANOVA with Šídák post hoc test.
    Universal Plus Mrna Seq Library Construction Kit, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/universal+plus+mrna-seq+library+construction+kit/universal+plus+mrna+kit/bio_rxiv__2024__07__29__605660-390-6-12
    Average 90 stars, based on 1 article reviews
    universal plus mrna-seq library construction kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Plk4 overexpression promotes centriole overduplication. Structured Illumination Microscopy (SIM) images of RPE-1 cells with endogenous and overexpressed Plk4. Plk4, magenta and centrioles, green. Scale bar 0.5 μm. (B) Unk is elevated upon Plk4 overexpression. Left panel – MA plot of mRNA levels based on RNA seq of RPE-1 cells with overexpressed Plk4, 16 hours after overexpression. PLK4 and UNK transcripts are elevated by 1.5- and 1.4-fold, respectively. Right panel – frequencies of PLK4 and UNK mRNAs in RPE-1 cells with endogenous and overexpressed Plk4. Graph values expressed as the means of biological replicates and SD. P values were determined using one-way ANOVA with Šídák post hoc test. (C) Unk protein is elevated at the centrosome by Plk4 overexpression. Left panels – SIM images of endogenous Unk in RPE-1 cells with endogenous and overexpressed Plk4. Unk, magenta and centrioles, green. Scale bar, 5 μm. Insets scale bar, 1 μm. Middle panels – 8 μm radial fluorescence intensity and corresponding ratio quantification of Unk using Centrin as the centrosome centroid. Right panel – centrosomal Unk fluorescence intensity based on binned central 2 μm. Graph values expressed as the means of biological replicates and SD. P value was determined using an unpaired two-tailed t test. (D) Unk is required for Plk4-induced centriole overduplication. Left panels – SIM images of RPE-1 cells showing centrioles in Unk depleted cells with endogenous and overexpressed Plk4. Unk, magenta and centrioles, green. Scale bar, 0.5 μm. Middle panel – frequency of cells with centriole overduplication. Right panel – number of cells with greater than, equal to, and less than four centrioles. Graph values expressed as the means of biological replicates and SD. P values were determined using one-way ANOVA with Šídák post hoc test.

    Journal: bioRxiv

    Article Title: The Unkempt RNA binding protein reveals a local translation program in centriole overduplication

    doi: 10.1101/2024.07.29.605660

    Figure Lengend Snippet: (A) Plk4 overexpression promotes centriole overduplication. Structured Illumination Microscopy (SIM) images of RPE-1 cells with endogenous and overexpressed Plk4. Plk4, magenta and centrioles, green. Scale bar 0.5 μm. (B) Unk is elevated upon Plk4 overexpression. Left panel – MA plot of mRNA levels based on RNA seq of RPE-1 cells with overexpressed Plk4, 16 hours after overexpression. PLK4 and UNK transcripts are elevated by 1.5- and 1.4-fold, respectively. Right panel – frequencies of PLK4 and UNK mRNAs in RPE-1 cells with endogenous and overexpressed Plk4. Graph values expressed as the means of biological replicates and SD. P values were determined using one-way ANOVA with Šídák post hoc test. (C) Unk protein is elevated at the centrosome by Plk4 overexpression. Left panels – SIM images of endogenous Unk in RPE-1 cells with endogenous and overexpressed Plk4. Unk, magenta and centrioles, green. Scale bar, 5 μm. Insets scale bar, 1 μm. Middle panels – 8 μm radial fluorescence intensity and corresponding ratio quantification of Unk using Centrin as the centrosome centroid. Right panel – centrosomal Unk fluorescence intensity based on binned central 2 μm. Graph values expressed as the means of biological replicates and SD. P value was determined using an unpaired two-tailed t test. (D) Unk is required for Plk4-induced centriole overduplication. Left panels – SIM images of RPE-1 cells showing centrioles in Unk depleted cells with endogenous and overexpressed Plk4. Unk, magenta and centrioles, green. Scale bar, 0.5 μm. Middle panel – frequency of cells with centriole overduplication. Right panel – number of cells with greater than, equal to, and less than four centrioles. Graph values expressed as the means of biological replicates and SD. P values were determined using one-way ANOVA with Šídák post hoc test.

    Article Snippet: Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508) and sequenced on an Illumina NovaSEQ 6000 sequencer by the Genomics and Microarray shared resource at the University of Colorado Cancer Center.

    Techniques: Over Expression, Microscopy, RNA Sequencing Assay, Fluorescence, Two Tailed Test